The results showed that OTUB2 was slightly expressed in SHEE cells, and was mainly located in the cytoplasm, while in ESCC tissues, OTUB2 was strongly expressed in the cytoplasm, and there was a some expression in the nucleus, which was consistent with the expression and immunohistochemical results retrieved from the online databases (Fig. 7A). Here, OTUB2 is linked to esophageal squamous cell carcinoma.