This study first analyzed the expression of OTUB2 in esophageal squamous carcinoma and its relationship with YAP1/TAZ via bioinformatics analysis, and combined with immunohistochemical staining of paraffin tissue samples from ESCC patients and their associated clinicopathological parameters, we explored the expression of OTUB2 and YAP1/TAZ in ESCC and their relationship with relevant clinical parameters. This evidence concerns the gene WWTR1 and esophageal squamous cell carcinoma.