The results showed that the protein expression levels of YAP1, TAZ and CTGF were significantly decreased after OTUB2 knockdown in ESCC cell lines (P < 0.001);but the mRNA expression levels of YAP1, TAZ and CTGF were not significantly decreased after OTUB2 knockdown in ESCC cell lines (Fig. 10). The gene discussed is WWTR1; the disease is esophageal squamous cell carcinoma.