The results of the wound healing experiment showed that there was no significant difference in the width and area of wound healing between KYSE150 and shNC, but in KYSE150 cell line, the width and area of wound healing in shOTUB2#1 group were larger than those in controls, and the healing rate was lower, indicating a down-regulation of OTUB2 expression in KYSE150 can reduce the migration ability of tumor cells (Fig. 9A, B). The gene discussed is OTUB2; the disease is neoplasm.