To investigate the mechanisms by which IL-7 modulates the CD127 secretion by CD8+ T cells, 104 of purified peripheral CD8+ T cells from fourteen patient with primary cutaneous melanoma were stimulated with recombinant human IL-7 (10 ng/mL) for 48 h in the presence of anti-CD3/CD28, along with either JAK inhibitor (10 μmol/L), STAT5 inhibitor (250 μmol/L), or PI3K inhibitor (25 μmol/L). The gene discussed is CD8A; the disease is cutaneous melanoma.