Although we cannot exclude that lower bacterial loads in MRP8Cre+Casp1flox mice could influence the levels of IL-1β measured in BALFs, those results suggest that neutrophil Caspase-1 is also a contributor of IL-1β production upon PAO1, PAO1ΔExoS and PP34ExoUS142A infections (Fig 5D and 5G). Here, IL1B is linked to infection.