Strikingly, neutrophil pyroptosis and IL-1β release were not modified by the use of EGTA upon infection with PP34ExoUS142A or PAO1ΔExoS pyroptotic strains, suggesting that Calcium fluxes are important players of histone citrullination and DNA decondensation downstream of inflammasome activation in neutrophils (S4C and S4D Fig). Here, IL1B is linked to infection.