The role of NLRC4 in P. aeruginosa-induced neutrophil cell death is confined to Caspase-1-dependent pyroptosis because infection of WT and Nlrc4-/- BMNs with P. aeruginosa strains that trigger Caspase-1-independent neutrophil lysis (PP34) resulted in similar LDH release levels, akin to our earlier results in Casp1-/- and GsdmD-/- neutrophils (Figs 1F and 2A). Here, CASP1 is linked to infection.