Among the different tested neutrophil genotypes, significant resistance to pyroptotic cell lysis (LDH release) was only observed in Casp1-/-, Casp1-/-Casp11-/-, Nlrc4-/- and ASC-/- BMNs upon infection with P. aeruginosa pyroptotic strains PAO1ΔExoS and PP34ΔExoU (S2A Fig). The gene discussed is NLRC4; the disease is infection.