Univariate analyses revealed that pT stage, pN stage, venous invasion (yes or no), Ki-67–positive rate (>50% yes or no), tumor size (>3cm yes or no), pathological differentiation (highly-moderately or poorly differentiation), Lauren type (intestinal or diffuse-mixed), and serum AFP levels (20–300 ng/ml vs. 300–1,000 ng/ml vs. >1,000 ng/ml) were prognostic factors for AFP-positive GC (P < 0.05, Table 3). The gene discussed is MKI67; the disease is neoplasm.