CD8A and neoplasm: The difference in the number of total and OVA-specific CD8+ T cells in tumors was confirmed by immunofluorescence (IF) analysis by staining with fluorescence-labelled OVA dextramers one tumor from animals that received either EcN or EcN(lpp-OVA) (OVA+ T cells/mm2: P = 0.0022; CD8+ T cells/mm2: P > 0.05; CD8+ T cellsEcN(lpp-OVA)//CD8+ T cellsEcN= 1.05; OVA-specific CD8+ T cellsEcN(lpp-OVA)//OVA-specific CD8+ T cellsEcN = 2.3) (Figure 3).