The expression of ARHGAP4 was highly correlated with the infiltration of CD4+ T cells in CRC and with dendritic cells in READ (Figure 4A).In colon cancer, after ARHGAP4 gene knockout, the levels of B cells, macrophages, neutrophils, and dendritic cells are downregulated. Here, ARHGAP4 is linked to malignant colon neoplasm.