Most of these studies have employed panCK to define the tumor compartment, along with immune markers such as CD45, CD3, CD8, and CD68 to define different immune compartments for ROI segmentation (50–52), Among the advantages of DSP profiling in these tumor types was the possibility to interrogate a high number of biomarkers into different spatial types provided by the VM, such as the expression of PD-L1 in tumor cells (defined by panCK-positive expression) or macrophages (defined by CD68 expression). Here, CD68 is linked to neoplasm.