S100B and melanoma: performed digital spatial profiling using a 44-plex antibody cocktail to search for protein expression that could potentially be used to predict response to immune therapy in melanoma, using a cocktail of antibodies against S100 and HMB45, CD68, and CD45, to identify and segment ROI in tumor cells (S100/HMB45+), macrophages (CD68+), and leukocytes (CD45+) (62).