IHC analysis confirmed that CRTAC1 and FOXO3 proteins were highly expressed in adjacent non-cancerous bladder tissues, 59.26% and 51.85%, respectively, while GSDMB and AIM2 proteins were highly expressed in BLCA tissues, 59.26% and 85.19%, respectively (Figure 6). The gene discussed is FOXO3; the disease is bladder transitional cell carcinoma.