Next, we validated the expression level by western blot, where we found that Notch1 and GAPDH were downregulated in CML, while Ikaros, HIF1α, p53, SIRT1, TNFα, and Foxo-3a were upregulated in CML (β-actin as an internal control), as shown in Figures 4A–D. The gene discussed is ACTB; the disease is chronic myelogenous leukemia, BCR-ABL1 positive.