Enzyme-linked immunosorbent assay blood indicators were used to objectively show the relationship between NPY and CFS symptoms of fatigue, sleep, anxiety, and depression after PLWNT intervention, and the findings were consistent with those of some previous studies (75, 76), which showed that different forms of exercise can reduce the level of expression of NPY and inhibit the development of the disease. The gene discussed is NPY; the disease is Anxiety.