Based on these studies, here, we built chronic visceral hyperalgesia models and administered NUCB2/Nesfatin-1 through different approaches; then, we compared the regulating effects of NUCB2/Nesfatin-1 on chronic visceral hyperalgesia sensitivity, laying the foundation for in-depth exploration of the relationship between NUCB2/Nesfatin-1 and IBS. The gene discussed is NUCB2; the disease is irritable bowel syndrome.