Figure 6(c) revealed significantly elevated PD-L1 expression in samples from subtype 4. We then analyzed the correlation between the expression level of RAD54B and TMB and MSI in the identified HCC subtypes. It was obvious that the expression of RAD54B in subtype 4 was significantly positively correlated with TMB and MSI but not in other subtypes (Supplementary Fig. 7F-G). The gene discussed is CD274; the disease is hepatocellular carcinoma.