For confirming the in vitro data, we surgically established IDD in mice and demonstrated the therapeutic effects of BMSC-EVs on IDD; additionally, we showed that the mechanism by which the circ_0050205/miR-665/GPX4 axis mediated the inhibitory effects of BMSC-EVs in vitro was also relevant for the effects BMSC-EVs in vivo. This evidence concerns the gene GPX4 and intervertebral disk degenerative disorder.