CD274 and neoplasm: Flow cytometry analysis of the immune cells in the spleens of tumor-bearing mice showed that the proportion of CD3+ T cells in the IR group was higher than that in the NR group, and more CD3+ T cells were detected in the spleens of PD-L1−/− mice, although the proportions of intrasplenic CD3+ cells in IR groups were still less than that of non-tumor control group (Figures 3C and 3E).