To detect the synergistic effect of IR and PD-L1 deficiency in ATAE, we constructed a syngeneic tumor model in PD-L1 wt mice and PD-L1−/− mice by subcutaneously inoculating Lewis lung carcinoma (LLC) cells into the right flank (as the primary tumor) and left flank (as the distal tumor) of a mouse. Here, CD274 is linked to neoplasm.