In summary, fecal samples from 15 type 2 diabetes patients and 15 type 2 diabetes patients treated with probiotics were collected for targeted metabolomics detection of short-chain fatty acids, while fecal samples from 15 type 2 diabetes patients treated with GLP-1 were collected for metabolic analysis of short-chain fatty acids. The gene discussed is GLP1R; the disease is type 2 diabetes mellitus.