However, in the GO enrichment analysis of differential genes in colon cancer, we also enriched some genes that are less studied or even never studied in colon cancer, such as POLD2, CDC16, PRIM2, etc. At the same time, some of the pathways of KEGG enrichment are relatively rare in colon cancer, such as the AGE-RAGE signal pathway in diabetic complications, oxytocin signal pathway, and coronavirus disease COVID-19. This evidence concerns the gene CDC16 and malignant colon neoplasm.