To further elucidate the functional role of O-GlcNAcylation in mesenchymal pancreatic cancer cells ferroptosis, stable GFPT1 (GFPT1 shRNA1 and shRNA2) and OGT (OGT shRNA2 and shRNA3) knockdown cell clones were established with high silencing efficiency confirmed by western blotting (Figure S2A-B). Here, GFPT1 is linked to pancreatic neoplasm.