CD79A and tetanus: To assess the humoral immune response, we measured the antigen-specific IgA and IgG levels via ELISA in mice immunized with either choleratoxin or pediarix (immunization against diphtheria, tetanus, and pertussis), and there were no differences in the levels of CTB-IgA, CTB-IgG, and TT-IgG between GF-immunized and those supplemented with HMO for 14 days and immunized (Figures 9A–C).