As expected, PC tumor cells showed enhanced expression of CD44 (p = 0.0007) and CD133 (p = 0.0224) together with significantly reduced proliferation rate (p<0.0001) as compared to primary tumor cells, whereas the upregulation ofc-Myc was not significant (p = 0.2826) (Figure 4B). This evidence concerns the gene CD44 and pachyonychia congenita.