Furthermore, nucleofected OT-I cells displayed subtle differences in cytokine producing ability on day 7 as compared to NTC OT-I cells (Supplementary Figure 5), whereas there was a slight decrease in the frequency of IFN-γ+ cells (but not IFN-γ+ TNF-α+ cells) >40 days after infection (Supplementary Figure 6). The gene discussed is IFNG; the disease is infection.