A total of 30 frequently mutated genes (FMGs) were identified in the CRC patients from TCGA, consisting APC (78%), TP53 (61%), TTN (48%), KRAS (43%), SYNE1 (28%), MUC16 (25%), PIK3CA (25%), FAT4 (22%), RYR2 (19%), ZFHX4 (19%), OBSCN (18%), DNAH5 (17%), DNAH11 (17%), LRP1B (16%), PCLO (16%), ABCA13 (16%), FBXW7 (16%), CSMD1 (15%), FLG (15%), CSMD3 (15%), USH2A (14%), FAT3 (14%), RYR1 (14%), ADGRV1 (14%), LRP2 (14%), MUC4 (14%), SMAD4 (13%), RYR3 (13%), MUC5B (13%), NEB (13%; Figure 7A). Here, SMAD4 is linked to colorectal carcinoma.