We noted that postoperative DTC patients with lymphatic metastasis also presented with an increased percentage of B cells and Tregs in their blood when compared with those without metastasis (P = 0.040, P = 0.044), while the percentage of CD4+ T cells and the absolute number of Th1 cells significantly decreased in these samples (P = 0.024, P = 0.037, Tables 2, 3). This evidence concerns the gene CD4 and Lymphatic Metastasis.