TNF and alkaline phosphatase measurement: We first used cerulein to treat AR42J acinar cells to construct an AP cell model and extract the cell culture supernatant and used an ELISA kit to detect pro-inflammatory cytokines (TNF-α and IL-6) and myocardial injury markers (LDH and CK-MB); the results showed that the contents of TNF-α, IL-6, CK-MB, and LDH in AP cells increased significantly, indicating that the AP cell model was successfully constructed.