MAP3K11 and ovarian carcinoma: To study the regulation of MLK3 during ovarian cancer cell cycle progression, SKOV3 ovarian cancer cells were arrested in mitosis by treatment with nocodazole (1 μg/ml) for 16 h, as indicated by the increase in phospho-Histone H3 (Ser10; p-Histone H3) protein levels (Fig. 1A) and by flow cytometry analysis (Fig. S1).