ESR1 and Decreased total granulocyte count: To explore whether E2 induced granulocytopenia in c-mybhyper zebrafish is ER-dependent, we knocked down or activated the classical nuclear receptors (nER: esr1, esr2a and esr2b) and membrane receptor (mER: gper1) through MO micro-injection or reported agonist (ERα agonist PPT, ERβ agonist DPN and gpr30 agonist G1), respectively.