In addition, multivariate analysis showed that SDHAF2, MRPL11, COX8A, serum carbohydrate antigen 19-9 (CA19-9), tumor size and lymph node metastasis are independent factors for overall survival (OS) of ICC patients with adjuvant TACE. This evidence concerns the gene SDHAF2 and metastatic malignant neoplasm in the lymph nodes.