To investigate the effects and selectivity of HA-CPNs in glioma proliferation in vivo, zebrafish embryos at 48hpf were injected with either FACS- derived CD44 + and/or CD44- or heterogeneous U-251 MG cells or patient derived cell lines in the presence of HA-CPNs and/or vehicle control and analyzed up to 6dpi populations (Fig. S6b). This evidence concerns the gene CD44 and central nervous system cancer.