PDX1 and Hyperglycemia: To test whether thiosulfate and hTST could rescue alterations of the pronephros caused by hyperglycemia, we induced hyperglycemia in zebrafish embryos utilizing a knockdown strategy targeting the transcription factor Pancreatic and duodenal homeobox 1 (Pdx1), which is essential for pancreatic cell development, using the morpholino technology31.