The remaining 67 (11.2%), 20 (3.33%) in the second trimester, 19 (3.17%) in the third trimester, 14 (2.33% %) in the first trimester, and 14 (2.33% %) non-pregnant women were excluded for the following reasons: positive serological tests (HIV, HBsAg, HCV, Syphilis and CRP), history of chronic diseases, recent blood transfusion and donation, obesity, and high blood pressure. Here, CRP is linked to obesity due to melanocortin 4 receptor deficiency.