CRP and syphilis: The remaining 67 (11.2%), 20 (3.33%) in the second trimester, 19 (3.17%) in the third trimester, 14 (2.33% %) in the first trimester, and 14 (2.33% %) non-pregnant women were excluded for the following reasons: positive serological tests (HIV, HBsAg, HCV, Syphilis and CRP), history of chronic diseases, recent blood transfusion and donation, obesity, and high blood pressure.