In addition, alterations in CD44, ID1, and TROP2, expression were also validated in the other CRC cell line, HT29 and its corresponding ATF2-KO clones (Fig. 2D and Supplementary Fig. 2G), with SOX9 being unchanged and VCAN and TWIST1 being undetectable. The gene discussed is TWIST1; the disease is colorectal carcinoma.