During 40 min of LCA occlusion without reperfusion, NC attenuated ischemic myocardial infarct size by 50% compared to normothermic control as defined by TTC-blue staining (Figure 5) and significantly decreased CP levels of cTnT, cfDNA and HMGB1 (Figure 6). This evidence concerns the gene HMGB1 and myocardial infarction.