To evaluate whether SLAMF8 can modulate the expression of TLR4 and SLAMF9 and, therefore, influence the observed phenotype in SLAMF8-deficient mice, we analyzed their expression in pMø by RT-PCR after treatment with IFNγ (100 U/ml for 16 h) and infection with S. typhimurium at different time points (Figure 7). This evidence concerns the gene TLR4 and infection.