RASSF1 and neoplasm: For the tumor samples from patients with LUAD, the sensitivity of the SHOX2 promoter methylation assay was slightly higher than that of RASSF1A, while the AUC of the SHOX2 and RASSF1A combined promoter methylation assay was significantly higher than those of individual SHOX2 and RASSF1A assays, respectively (DeLong test, P < 0.05) (Table 2; Figure 2A).