Therefore updated guidelines from the TP53 network of ERIC (European Research Initiative on CLL- www.ericcll.org) suggest a threshold of 10% allelic burden for reporting mutations detected by NGS segregating these into high burden (≥10 variant allele frequency (VAF)) and low burden (<10% VAF) mutation (6). This evidence concerns the gene TP53 and B-cell chronic lymphocytic leukemia.