Briefly, NSCLC cell lines (A549 and NCI-H460 lung adenocarcinoma) or lung epithelial cell line BEAS-2B were cultured in combination with MRC-5 lung embryonic fibroblasts, and THP-1 monocytes in the DMEM media supplemented with 10% FBS and hence named as A549+MRC-5+THP-1 (AMT spheroids) and NCI-H460+MRC-5+THP-1 (HMT spheroids), and BEAS-2B+MRC-5+THP-1 (BMT spheroids) (Figures 1A–C). Here, GLI2 is linked to lung adenocarcinoma.