While measuring changes in Ca2+ dynamics in advanced stages of T1D in isolated mouse islets is technically challenging, future studies utilizing NOD pancreas slices from animals with advanced disease, coupled with immunocytochemical labeling of β-cells and T-cells, couldp provide insight into further alterations in Cx36 coupling and Ca2+ dynamics in T1D progression. Here, GJD2 is linked to type 1 diabetes mellitus.