Furthermore, we analyzed the prognostic value of DNA methylation expression levels of USP45 in pan-cancer, the results showed that cg08792129, cg10139443, cg13363689, cg21257892, cg16486502, cg26484090, cg25198784, cg25309811, cg27317327, cg10970399, cg13809693, and cg17635724 from USP45 had the highest DNA methylation levels and significant prognostic value (likelihood ratio (LR) test p-value < 0.05) in different types of tumors (Supplementary Table S1). The gene discussed is USP45; the disease is cancer.