To further validate our conclusions, we analyzed the protein expression levels of USP45 in 12 types of tumor and their paired paracancerous tissues, including COADREAD, ESCA, GBMLGG, LIHC, LUAD, LUSC, oral squamous cell carcinoma (OSCC), PAAD, PRAD, renal cell carcinoma (RCC), triple-negative breast cancer (TNBC), non-triple-negative breast cancer (non-TNBC) using immunohistochemistry. This evidence concerns the gene USP45 and pancreatic adenocarcinoma.