MLKL and Hepatic fibrosis: Furthermore, in the classical CCl4 induced mice liver fibrosis model, both the western blot analysis (Figure 1D-E) and quantitative RT-PCR analysis (Figure 1F) confirmed that MLKL was significantly upregulated in both the protein and mRNA levels, accompanied by the significant upregulation of a number of fibrosis markers, comparing to the vehicle control group.