Considering hepatocyte damage is the first step in liver fibrosis, we generated adeno-associated virus (AAV) type 8 carrying Mlkl shRNA (AAV8-shMlkl) or scramble shRNA (AAV8-scramble) under the control of the hepatocyte-specific thyroid hormone-binding globulin (TBG) promoter. The gene discussed is MLKL; the disease is Hepatic fibrosis.