BRD4 and carcinoma: In NUT midline carcinoma cells, A-485 reduces histone acetylation, disrupts BRD4-NUT binding, and down-regulates the expression of BRD4-NUT target genes including MYC and TP63. A-485 thereby strongly induces NUT midline carcinoma cell differentiation, cell cycle arrest and apoptosis 103 (Table 2).