YAP1 and sarcoma: To test whether α-actinin-binding is involved in the CLP36-mediated regulation of YAP1 expression, we expressed a 3xflag-tagged α-actinin-binding defective CLP36 mutant (3fl-ABD) 31 or 3xflag-tagged wild type CLP36 (3fl-CLP36) as a positive control in CLP36 KO p53 deficient sarcoma cells.