PDLIM1 and sarcoma: To do this, we increased YAP1 expression in CLP36 KO p53 deficient sarcoma cells by infecting them with lentiviral vectors encoding 3xflag-tagged YAP1 (3fl-YAP1) (Figure 8A, lane 4) or 3xflag (3fl) (Figure 8A, lane 3) as a control and tested the effects on cell behavior.