Pituitary adenoma cells Att20 were treated by TMZ and normal control cells were sequenced and analyzed by EIF5A, H3F3C, PTBP1, FKBP1C, CARS, PSAT1, GPS2, PGAM4, RNF112, LMNB1 was highly expressed in sensitive cell lines treated with TMZ, while SLC27A1, IFI30, CDC42BPG, KIFC1, P2RX3, EDA2R, AK1, FETUB, TMEM43, RPS10-NUDT3 was highly expressed in control cells (Figures 2B,D). The gene discussed is PGAM4; the disease is pituitary gland adenoma.