As PPP2R5D is an isoform of the subunit family B56 of the enzyme serine/threonine-protein phosphatase 2A (PP2A) involved in HCC, multiple diseases, and cellular pathways [15, 16], we proceeded to investigate whether the interaction and colocalization between PPP2R5D and HCV NS5B affected the activity of PP2A. The gene discussed is PPP2R5D; the disease is hepatocellular carcinoma.