As shown in Fig. 2A–D, based on the analysis of sample type, molecular subtype, cancer stage and lymph node metastasis, the expression of ENO1 in BLCA patients was dramatically higher than that in normal controls, while there is no significant difference in the expression of ENO2 and ENO3 among the clinical variables (Fig. 2E–L). This evidence concerns the gene ENO2 and bladder transitional cell carcinoma.