CD8A and melanoma: Flow cytometry analysis showed that the numbers of PD-1+ CD8+ TILs were significantly lower in tumors expressing HRSS345D compared to those expressing HRSWT or HRSS345A (Fig. 2a), consistent with the negative correlation of p-HRSS345 and CD8+ TILs in melanoma patients shown by IHC (Fig. 1e–g).