We undertook a two-generation cross of the Tc1 mouse model of DS (O'Doherty et al., 2005), which contains a freely segregating copy of Hsa21 (but not a functional additional copy of APP) (Gribble et al., 2013), with the AppNL-F model to generate four genotypes of mice (WT, Tc1, AppNL-F/NL-F, and Tc1;AppNL-F/NL-F). This evidence concerns the gene APP and Dravet syndrome.