To identify the role of autophagy in the regulation of the skin barrier in AD, we crossed Atg7-floxed mice (referred to as Atg7fl/fl mice hereafter) (34) with K14-Cre transgenic mice to generate mice with selective ablation of Atg7 in keratinocytes (referred to as K14Cre Atg7fl/fl mice hereafter). Here, KRT14 is linked to Alzheimer disease.