Among the recurrent gene mutations in children with 11q23/KMT2A‐rearranged AML, those with higher VAFs (>0.3) gene mutations were U2AF1, CCND3, STAG2, TET2, RYR1, and MKI67, which presumably occur earlier during leukemogenesis. This evidence concerns the gene RYR1 and acute myeloid leukemia.