A high-risk score is associated with spliceosome regulation, cell cycle, base excision repair, RNA degradation, oocyte meiosis, ubiquitin-mediated proteolysis, pyrimidine metabolism, DNA replication, homologous recombination, mismatch repair, nucleotide excision repair, cytosolic DNA sensing pathway, endocytosis, neurotrophin signaling pathway, basal transcription factors, pathways in cancer, WNT signaling pathway, etc. (Figure S5 and Table 5). The gene discussed is BDNF; the disease is cancer.