Tumor cells were infected with vaccinia virus, an OV, coding CD19t that expressed de novo CD19 on the cell surface before virus-mediated tumor lysis; it promotes the targeting of tumor cells by co-cultured CD19-CAR-T cells, thereby inducing the secretion of cytokines, showing potent cytolytic activity against infected tumors, confirming the OV19t promoted tumor control by CD19-CARt cells in several mouse tumor models (92). The gene discussed is CD19; the disease is neoplasm.