The thyroid cancer cell lines were treated with 1640, nontargeted contrast agent (20 pmol/mL), targeted contrast agent (20 pmol/mL), and adriamycin (0.5 umol/mL) for 72 hours, and then the effects on caspase-3, BAX, and Bcl-2 expression were observed. The gene discussed is CASP3; the disease is thyroid cancer.